The labscan3d and labscan 100 systems are based on luminex xmap technology and use luminex xponent software for data acquisition. Like the luminex 200 and flexmap 3d systems, the magpix system uses xponent software, offering performance and user interaction similar to these more. Luminex 200 system user manual the following label appears on the back of the luminex 200 instrument. I now want to use an aliquot of the original, unadjusted samples. One led identifies the analyte that is being detected and, the second led. A access doors must be closed while operating the luminex 200 analyzer. Manual, magpix installation and hardware user ruo, english nxtag enabled posted on october 1, 2015 november 24, 2015 by luminex corporation manual, magpix installation and hardware user ruo, english nxtag enabled. Turn on instrument there are two power switches, one on the back and one on the front.
Next, contact luminex technical support for a recycled returned materials authorization rma number. For luminex 100200 and flexmap 3d instruments initiate the startup protocol to warm up the lasers for at least 30 minutes. For the past 10 years, two instrument systems for evaluating assays based on xmap technology, the luminex 200 and flexmap 3d systems, have gained wide acceptance for use in applications including protein expression profiling, focused gene. Refer to the decontaminating the luminex 200 analyzer for return shipment section in the safety chapter of the luminex 200 system manual. Luminex 200 system and luminex xy platform the luminex 200 system provides a complete mid to high range solution for rapid, accurate biomarker quantification. Gpp can be run using a luminex 100200 or magpix system. Biological heat blue indicator light the blue light above the luminex 20 0 analyzer sample arm indicates. Manual, magpix user quick guide luminex corporation. This manual provides stepbystep instructions on how to start your magpix system for the first time, how to perform daily operations, and how to properly maintain the instrument. Page 8 when you turn on the luminex magpix system, the following start up indicators occur. The luminex magpix multiplex system brings you easy to operate, easy to maintain, economical and compact power for your elisa assay needs. Oct 01, 2015 manual, magpix installation and hardware user ruo, english nxtag enabled posted on october 1, 2015 november 24, 2015 by luminex corporation manual, magpix installation and hardware user ruo, english nxtag enabled. Manual, luminex 200 user ivd, english luminex corporation. Remove the plastic shield that covers the sample probe area.
Analytespecific antibodies are precoated onto magnetic microparticles embedded with. With more than 11,000 units sold globally to date, the luminex 100200 is the established standard in multiplexing instrumentation. This multiplex, fluorescent, beadbased system offers a simple and efficient alternative to ifa and elisa testing. At this time the following items are accepted by the lcrf for repair. The luminex xy platform luminex xyp complements the luminex 100 and 200 systems by automating the sequential positioning of each well of a microtiter plate. The luminex features innovative computer technology and advanced software science to provide something entirely new.
Typically used in bead coupling reactions, this magnet can quickly separate one ml of magplex microspheres from solution and is a helpful addition to any xmap bench top. Luminex magpix quick guide rndsylu2945 for full details, reference the software manual 1. Magpix clinical diagnostics instrument is a simple, affordable, and compact solution for multiplexing. Powerful more than 125 and growing milliplex magnetic beadbased assay kitsthe largest offering of customizable magnetic bead immunoassay panels for the magpix instrument. The luminex 200 detects up to 100 protein or nucleic acid analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. For the past 10 years, two instrument systems for evaluating assays based on xmap technology, the luminex 200 and flexmap 3d systems, have gained wide acceptance for use in applications including protein expression profiling, focused gene expression profiling, and disease testing. The second is a fluorescent imaging and flow cytometrybased instruments, the luminex magpix and luminex 200flexmap 3d analyzer, respectively, which integrates key xmap detection components such as light emitting diodes leds and a ccd camera or lasers, optics, advanced fluidics and highspeed digital signal processors. Use our product documents resource to find product manuals, help guides, coq, msds and more. This compact multiplexing unit performs up to 50 different tests in a single reaction volume and reads a 96wellplate in just 60 minutes. The bioplex magpix multiplex reader is now discontinued.
The new magpix multiplexing system thermo fisher scientific. Lowconcentration magnetic magplex microspheres which eliminate the need for expensive filter plates and come in a working concentration better suited for assay development and lowthroughput applications. When performing routine maintenance, turn power to the luminex 200 analyzer off. The new magpix multiplexing system thermo fisher scientific us.
L of assay buffer to each of the 16 wells and read the plate with the luminex magpix instrument. The magpix uses mush less fluid volume, making it easier to. Luminex multiplexing instruments life science research merck. Magpix features selfcleaning routines and magnetic bead compatibility with magplex beads, making the. Luminex 200 system user manual xmap technology 1 4 pn 890000200109 rev. Page 1 lumnex luminex 200 system user manual page 2 surrey kt 17 1ej, england luminex corporation luminex reserves the right to modify its products and services at any time. Luminex 200multiplexing instrument from luminex selectscience.
When performing routine maintenance, turn power to the luminex 200 analyzer off and disconnect the power cord. Jul 06, 2012 recent improvements by luminex include. The most important thing about this writer is whats invisible. Using the labscan systems with our labtype and labscreen family of products allows the user to make assignments of hla antibodies as well as hla typing with the analysis support of hla fusion. A10881 the luminex multiplex basic training program provides new users of the luminex 200 or magpix system with training in xmap technology, xponent software, luminex system hardware, maintenance, tips for troubleshooting and firsthand experience setting up and running a luminex multiplex protein assay. Bioplex cytokine assay instruction manual for technical service, call your local biorad office, or in the us, call biorad. This assay may be run alone or in combination with other select luminex singleplex kits. May 21, 2015 learn how to adjust the probe height on your luminex 200 instrument by following these step by step instructions. Magpix multiplexing instrument from luminex selectscience. Utilizing technology based in flow cytometry, this instrument is. Designed for easeofuse, xponent enables both new and advanced users to set up and run assays in a minimal amount of time.
Suitable for use with luminex 100200, flexmap 3d and magpix systems. Lowcost entry level platform with proven xmap technology. Although prepared to ensure accuracy, luminex assumes no liability for errors or omissions, or for any damages resulting from the application or use of this. The magpix multiplex system can process up to 50 tests within one sample volume and can read a 96well plate in 60 minutes, far more efficient than traditional elisa or western blotting that can eat up samples and time. Luminex 100200 for life science research standard for.
Alternatively, kits can be used with the luminex 100200, luminex flexmap 3d, or biorad bioplex, dual laser, flowbased sorting and detection platforms. We will also continue to provide service, support, accessories, and software for customers with the bioplex magpix multiplex reader. Ensure that the luminex machine is calibrated according to the manufacturers instructions. Watch video on luminex xmap technology as a luminex partner, we are a preferred distributor of the entire luminex portfolio of multiplexing instruments, including luminex 200, flexmap 3d and the magpix platforms, covering the full range of multiplexing capabilities. Select an antibody pair that meets your desired signal strength. May, 2015 the luminex 100200 system is the combination of three core xmap technologies. This affordable system can perform up to 50 tests simultaneously in a single reaction volume. This manual describes the features and functions of xponent for magpix. Luminex corporation luminex reserves the right to modify its products and services at any time. The luminex 200 is a multiplex particle analyzer suited for analysis of a variety of assays. Its selfcleaning routines and magnetic bead compatibility with magplex beads make magpix extremely easy to learn and use. Magpix system how to use protocol and batch function. Luminex 100200, luminex xyp xy platform, luminex sd sheath delivery system, magpix, flexmap 3d and optical platform.
The luminex is smaller and lighter, has an extraordinary degree of precision, and provides the finest writing experience ever possible. We will continue to provide premium luminex instrumentation. Sxga 1280 x 1024 with 32bit color pc specifications processor. Luminex 100 200 sets the standard for multiplexing, with over 11,000 units sold globally and more than fifty 510 kcleared assays. Clinical diagnostics instruments magpix multiplexing luminex.
Posted on august 7, 2014 november 24, 2015 by luminex corporation. Bioplex magpix multiplex reader life science research. Luminex logo home link clinical infectious disease coronavirus covid19 respiratory infection bloodstream infection. Luminex bio rad manual bioplex magpix or luminex magpix system, bioplex 200 or luminex 200 system, manuals and user guides for free. In addition to flowbased analyzers, magnetic beads can be read using the luminex magpix analyzer. Magpix system life science research milliporesigma. Magnetic separators for xmap assays luminex corporation. Luminex 100, luminex 200, flexmap 3d, magpix, bioplex luminex corporation vortex mixer major laboratory supplier mls. The reader combines two lasers, fluidics, and realtime digital signal processing to distinguish up to 100 different sets of colorcoded polystyrene beads, each bearing a different assay. Users can expect to see similar median fluorescence intensity mfi data from their assays, whether run on the magpix or luminex 200 system. All of our procartaplex assays are compatible with as all of our procartaplex assays are compatible with as little as 25 l of plasma or serum samples or 50 l of cell culture supernatant sample. The luminex magpix analyzer utilizes a magnet to hold the superparamagnetic microparticles in a monolayer while two spectrally distinct light. The first is xmap microspheres, a family of fluorescently dyed. The magpix is a compact, robust, cost effective multiplexing system based on ccd imaging technology.
Under no circumstances should you remove the luminex 200 analyzer cover. This protocol is a stepbystep method for setting up the xponent 3. The luminex 200 is the established standard in multiplexed instrumentation. Furthermore, the magpix instrument was designed to yield equivalent results to the luminex 200 instrument. Magpix is the simplest, most affordable, and most compact of luminex s xmap instruments. Conversion of a capture elisa to a luminex xmap assay using a. The second is a fluorescent imaging and flow cytometrybased instruments, the luminex magpix and luminex 200 flexmap 3d analyzer, respectively, which integrates key xmap detection components such as light emitting diodes leds and a ccd camera or lasers, optics, advanced fluidics and highspeed digital signal processors. Bioplex assays are beadbased assays that can be performed in a mixed array multiplexed. Adjust probe height if probe has been removed page 115 of xponent software manual a. Luminex technology employs microsphere beads, either polystyrene or paramagnetic, that are colorcoded.
We are currently moving from luminex 200 to magpix and the advantage is ease of use both systems use similar software, lower sample volume with the magpix 50 ul over 100 ul sample, and use of mylar plates over filter bottom plates has prevented clogging from complex samples. Magpix instrument doesnt require additional warm up. A magnet in the magpix analyzer captures and holds the magnetic beads in a monolayer, while two spectrally distinct lightemitting diodes leds illuminate the beads. Luminex 200 system life science research milliporesigma. Manual, luminex 200 user ivd, english downloads 296 total files 1 create date march 6, 2019 last updated march 6, 2019 download file action 890000200620. The panel uses magnetic beads, facilitating automation, decreasing handson time, and increasing throughput and precision. Luminex assay multiplex kits are designed for use with the luminex magpix ccd imager.
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